Literature analysis indicates that NLS-based skin studies are commonly applied to various oncological diseases. Two-dimensional and three-dimensional NLS imaging can be used to study skin tumors. In most cases, tumors appear as areas of increased chromatinization, more or less separated from the dermis. It is impossible to define the histological characteristics of a tumor solely based on NLS imaging.

For the differential diagnosis of skin tumors such as hemangiomas or melanomas, the ultra-microscopic scanning mode combined with spectral entropy analysis (SEA) can be successfully applied.

Malignant melanomas can be studied using NLS imaging. Melanomas in NLS studies are spindle-shaped, homogeneous in structure, and well-separated from surrounding tissues. We noted high accuracy in tumor size assessment, which was confirmed by comparison with data obtained from pre-surgical NLS studies and post-operative histological testing. According to other data, although tumors are well visualized in NLS graphic studies, a major problem is distinguishing the tumor parenchyma from the surrounding inflammatory infiltration, as they appear as a single, highly chromatinized structure. Therefore, the size of some tumors may be exaggerated in NLS studies. This is relevant to the study by Ulrich J. et al. The results show that the accuracy of tumor size assessment is significantly improved if tumors with extensive inflammatory infiltration or melanoma associated with nevi are excluded from the analysis when applying SEA.

Modern NLS technology with Biophilia Tracker allows for more accurate assessment of changes in various skin diseases; it not only improves diagnosis and allows for initiating treatment at the appropriate time, but also ensures controlled treatment efficacy.