3D NLS methods. Scans were performed using Biophilia Intruder with a software program that allows visualization of all bones, surrounding soft tissue, and lymph nodes. The NLS study begins with a full-body assessment and continues with detailed evaluation of the following body areas: head and neck; trunk and proximal shoulders; pelvis and proximal femur. The study protocol for each anatomical section included the following data: large-scale NLS scans in 3D mode in 3 mutually transverse planes; ultramicroscopic scan patterns in the region of interest analyzed with spectral entropy. The total time required for the study was approximately 30 minutes.

Bone scan method. Within 2 hours after intravenous injection of 740 Mbk 99mTs-Technephore, bone scans of the skeletal system were performed in anterior and posterior views using a dual-detector gamma camera according to standard methods. Images are captured using low-energy high-resolution collimated optics; the entire stage moves at 12-16 cm/min. The scanning process takes 15-20 minutes.

data analysis. An increase in the chromogenicity of lesions that is focal or diffuse (homogeneous or heterogeneous) of more than 4 points according to the Fleindler scale is considered a sign of metastatic disease during NLS imaging. Bone marrow changes are considered benign if they are located directly beneath the degenerated disc or near the articular surface. Spectral entropy analysis is critical in all cases.

Hyperspectral similarity (D<0,425) to the etalon «metastatic tumor subclone» is often referred to as a metastatic lesion. Validation of the spectral entropy analysis results was based on histological examination of rings received after ring biopsy of bone tissue in the region of interest. The results of spectral entropy analysis were confirmed histologically in 99.1% of cases.

The probability of metastatic disease (when a bone scan is performed) is estimated based on the location and number of hyperactive lesions, taking into account radiopharmaceutical overfixation therein.

Biophilia Intruder is a great value for all aspects of testing