1. The bladder wall in 3D ultramicroscopic imaging has a three-layer structure.

2. In the case of bladder cancer, common 2D NLS studies can reveal tumors if they exceed 5 mm in size. The tumor was smaller than 2 mm when studied by 3D ultramicroscopy. The form of tumor growth (exophytic, endophytic or combined) and the depth of its invasion into the wall can be detected and defined (influence of invasion rate on disease prognosis and treatment methods).

3. Diagnose the main problems that arise when the initial tumor invades the muscle layer. Ultramicroscopic scanning with spectral entropy analysis helps determine the depth of invasion when this occurs.

4. Familiarity with typical semiotics allows for correct diagnosis when performing NLS studies with Biophilia Intruder in the case of non-epithelial bladder tumors and urachal tumors.

5. The information recording of the medical history and the performance of the spectral entropy analysis allow a correct diagnosis in case of metastasis to the bladder wall of another primary localized tumor.

6. Ultramicroscopic studies using spectral entropy analysis can determine whether a tumor detected in the bladder originates from the bladder wall or is just a part of a tumor from another organ (colon, cervix, prostate, etc.) and enters the bladder wall.

7. For an appropriate diagnosis, it is necessary to use all available NLS investigations, taking into account the susceptibility of bladder tumors to multifocal growth.

8. Ultramicroscopic NLS studies are the most effective studies for bladder tumor diagnosis. The 3D reconstruction of the images increases the effectiveness of the diagnosis, it also provides a more informative representation of the detected changes, it offers the possibility to view the stored information.

9. In the case of bladder cancer, it is necessary to bear in mind the possibility of synchronous prostate cancer.